WST-8

CAS Register Number

193149-74-5

EC Number

693-016-8

IUPAC Name

sodium 4-[2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)tetrazol-2-ium-5-yl]benzene-1,3-disulfonate

Molecular Formula

C20H13N6NaO11S2

Molecular Weight

600.5 g/mol

SMILES

COC1=C(C=CC(=C1)[N+](=O)[O-])[N+]2=NC(=NN2C3=CC=C(C=C3)[N+](=O)[O-])C4=C(C=C(C=C4)S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+]

WST-8 (CAS 193149-74-5) is a next-generation, highly water-soluble tetrazolium salt and a superior chromogenic indicator for NADH. Essential for sensitive cell assays, it is reduced by viable cells to produce a soluble yellow formazan dye, providing a simple, non-destructive method to measure cellular metabolic activity.

Applications of WST-8 (Water-Soluble Tetrazolium 8)

This highly sensitive reagent is fundamental to modern cell proliferation and cytotoxicity assays, most notably as the core component of the widely used CCK-8 kit. WST-8 provides reliable and accurate quantification of viable cell numbers in critical life science applications, including drug screening, cancer research, and toxicity testing. Its unique water-soluble formazan product streamlines the process by eliminating the need for an extra dissolution step, allowing for simpler, high-throughput analysis compared to older salts like MTT.

Packaging

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